"Biochemical trapping of unidentified events" Page 8

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TABLE 10

Free amino acids content of Aledicago minima leaves in the third (H to L)

series of samples (D + 730 from UE)

H       I       J       K       L

Distance from
Epicenter (m): 0.5 3.8 6.0 8.8 15.4

Lys 0.14±0.047 0.07±0.024 0.10±0.034 0.05±0.017 0.07±0.024

Arg 0.47±0.052 0.33±0.040 0.23±0.025 0.06±0.007 0.35±0.038

His 0.09±0.020 0.18±0.040 0.12±0.026 0.15±0.033 0.12±0.030

Ser 0.15±0.03 0.29±0.06 0.30±0.06 0.30±0.06 0.32±0.07

Asp + Asn + Gly 0.54±0.09 1.01±0.18 0.91±0.16 0.77±0.14 0.82±0.15

GI + Gln 0.45±0.05 0.32±0.04 0.70±0.08 0.47±0.05 0.44±0.05

Thr 0.17±0.03 0.21±0.04 0.14±0.03 0.18±0.03 0.20±0.04

Ala 0.43±0.13 0.49±0.15 0.54±0.17 0.53±0.17 0.36±0.11

Pro 0.07±0.02 0.07±0.02 0.10±0.03 0.42±0.13 0.32±0.09

Val 0.25±0.05 0.21±0.04 0.17±0.03 0.17±0.03 0.13±0.02

Ile 0.07±0.012 0.12±0.02 0.13±0.02 0.14±0.024 0.10±0.02

Leu 0.08±0.02 0.06±0.02 0.11±0.03 0.10±0.03 0.08±0.02

Phe 0.15±0.04 0.24±0.06 0.31±0.08 0.20±0.04 0.25±0.05

Trp 0.21±0.04 0.29±0.06 0.36±0.08 0.29±0.06 0.23±0.05

noteworthy that lysine (Lys) is increased in both younger and older leaves from plants sampled near the epicenter (A), but with low significance (p = 0.097 and 0.067 respectively). In the second series (D + 40), Lysine again appears as more concentrated at the epicenter, point (C), by contrast with proline (Pro) and isoleucine (Ile), whose concentrations increase with increasing distances from the epicenter. Lysine concentrations are significantly different between C and D (p = 0.009) and C and G (p = 0.007). Proline is depressed in sample C relative to samples D-E (p = 0.06), F (p = 0.07), and G (p = 0.05). Isoleucine is slightly depressed in sample C versus samples F (p = 0.09) and G (p = 0.075). No such differences could be found in samples from the last series (D + 730 from UE).

Incidence of Time-Proximity to the UE on the Biochemical Variability

     The homogeneity of the distribution of biochemical parameters along the ecological axis may have been altered by an external factor. The variances calculated for the five samples at D + 40 and D + 730, respectively, have been compared by the "F" test. Significant differences have been noticed in the following 8 cases:

Chlorophyll A:     F=8.5     P(F)=0.03

Chlorophyll A/Pheophytin:     F=48.6     P(F)=0.001

Lutein:     F=11.5     P(F)=0.02

Glucose:     F=9.3     P(F)=0.03

TABLE 10
Free amino acids content of Aledicago minima leaves in the third (H to L)
series of samples (D + 730 from UE)

	H	I	J	K	L
Distance from Epicenter (m):	0.5	3.8	6.0	8.8	15.4

Lys	0.14±0.047	0.07±0.024	0.10±0.034	0.05±0.017	0.07±0.024
Arg	0.47±0.052	0.33±0.040	0.23±0.025	0.06±0.007	0.35±0.038
His	0.09±0.020	0.18±0.040	0.12±0.026	0.15±0.033	0.12±0.030
Ser	0.15±0.03	0.29±0.06	0.30±0.06	0.30±0.06	0.32±0.07
Asp + Asn + Gly	0.54±0.09	1.01±0.18	0.91±0.16	0.77±0.14	0.82±0.15
GI + Gln	0.45±0.05	0.32±0.04	0.70±0.08	0.47±0.05	0.44±0.05
Thr	0.17±0.03	0.21±0.04	0.14±0.03	0.18±0.03	0.20±0.04
Ala	0.43±0.13	0.49±0.15	0.54±0.17	0.53±0.17	0.36±0.11
Pro	0.07±0.02	0.07±0.02	0.10±0.03	0.42±0.13	0.32±0.09
Val	0.25±0.05	0.21±0.04	0.17±0.03	0.17±0.03	0.13±0.02
Ile	0.07±0.012	0.12±0.02	0.13±0.02	0.14±0.024	0.10±0.02
Leu	0.08±0.02	0.06±0.02	0.11±0.03	0.10±0.03	0.08±0.02
Phe	0.15±0.04	0.24±0.06	0.31±0.08	0.20±0.04	0.25±0.05
Trp	0.21±0.04	0.29±0.06	0.36±0.08	0.29±0.06	0.23±0.05


Chlorophyll A:	F=8.5	P(F)=0.03
Chlorophyll A/Pheophytin:	F=48.6	P(F)=0.001
Lutein:	F=11.5	P(F)=0.02
Glucose:	F=9.3	P(F)=0.03